Cl- and regulation of pH by MDCK-C11 cells

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Cl- and regulation of pH by MDCK-C11 cells.

The interaction between H(+) extrusion via H(+)-ATPase and Cl(-) conductance was studied in the C11 clone of MDCK cells, akin to the intercalated cells of the collecting duct. Cell pH (pHi) was measured by fluorescence microscopy using the fluorescein-derived probe BCECF-AM. Control recovery rate measured after a 20 mM NH(4)Cl acid pulse was 0.136 +/- 0.008 pH units/min (dpHi/dt) in Na(+) Ringe...

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Purinergic-induced signaling in C11-MDCK cells inhibits the secretory Na-K-Cl cotransporter.

Purinergic inhibition of Na-K-Cl cotransport has been noted in various renal epithelial cells derived from the collecting tubule, including Madin-Darby canine kidney (MDCK) cells. In recent studies, we have observed purinergic inhibition of Na-K-Cl cotransport in C11-MDCK subclones (alpha-intercalated-like cells). Interestingly, Na-K-Cl cotransport activity was also detected in C7-MDCK subclone...

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Shear stress-induced volume decrease in C11-MDCK cells by BK- / 4

Holtzclaw JD, Liu L, Grimm PR, Sansom SC. Shear stressinduced volume decrease in C11-MDCK cells by BK/ 4. Am J Physiol Renal Physiol 299: F507–F516, 2010. First published June 24, 2010; doi:10.1152/ajprenal.00222.2010.—Large-conductance, calcium-activated potassium channels (BK) are expressed in principal cells (PC) and intercalated cells (IC) in mammalian nephrons as BK/ 1 and BK/ 4, respectiv...

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Ammonium transport and pH regulation by K(+)-Cl(-) cotransporters.

The Na(+)-K(+)-Cl(-) cotransporters (NKCCs), which belong to the cation-Cl(-) cotransporter (CCC) family, are able to translocate NH4(+) across cell membranes. In this study, we have used the oocyte expression system to determine whether the K(+)-Cl(-) cotransporters (KCCs) can also transport NH4(+) and whether they play a role in pH regulation. Our results demonstrate that all of the CCCs exam...

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ژورنال

عنوان ژورنال: Brazilian Journal of Medical and Biological Research

سال: 2007

ISSN: 1414-431X

DOI: 10.1590/s0100-879x2006005000098